The taxonomic placement of Spanish isolates of Gremmeniella abietina var. abietina (Gaa) (race and biotype) was
determined, for the first time, using RAPD markers and comparison of RAPD patterns for Spanish isolates and those
originating from different regions of Europe and North America. Fifteen Spanish isolates of Gaa were isolated from
recently dead shoots of 10 Pinus halepensis trees. Additionally, 17 isolates of Gaa from Canada, Finland, Switzerland and
the USA, previously characterized according to race and biotype, were used. Five primers, previously reported to yield
readable, reproducible and polymorphic fragments, were used in PCR reactions. The results showed that the Spanish
isolates represent the European race and not the Alpine or northern biotypes. Spanish isolates appear to be clearly separated
from all other biotypes within the EU race and preliminary data suggest that Spanish isolates have low genetic variability.