We studied whether agrobiodiversity in home gardens reflects the cultural landscapes that embed them. We compared floristic composition, biomass and cover of trees in home gardens between the cultural landscapes on mountain slopes (MSL), small hills (SHL), and floodplains (FPL) in a segment of the Grijalva–Usumacinta watershed in the tropical lowlands of Tabasco, Mexico. We characterized the cultural landscapes based on information obtained through questionnaires, identified species and measured tree height and diameter at breast height in random samples of home gardens from two localities in each case. The cultural landscapes showed distinct land use combinations: MSL comprised subsistence agriculture, pasturelands and forests; SHL pasturelands, some secondary vegetation and industrial agriculture fields; and FPL mainly industrial agriculture fields and pasturelands. Total species richness was greater in MSL than in SHL and FPL. Mean species richness was greater in MSL and SHL (22.4 and 15.8 respectively) than in FPL (7.2), as was the mean number of individuals per home garden (98.2, 94.1 and 20.4. Dominant species in home gardens in FPL and SHL included particular secondary species for each landscape, whereas single or double occurrences of mature forest species were distinctive of home gardens in MSL. Mean biomass was greater in MSL than in SHL and FPL (37.1, 28.2 and 23.7 Mg C ha−1), as was tree cover (1.06, 0.95 and 0.76 m2/m2). We conclude that agrobiodiversity varies considerably among cultural landscapes and recommend the design of specific policies to enhance its conservation in each of them.